RESEARCH PAPER
Osteopontin enhances donor-specific alloreactivity of human peripheral blood mononuclear cells
1
Department of Clinical Immunology, Medical University, Warsaw, Poland
Corresponding author
Beata Kaleta
Department of Clinical Immunology, Medical University of Warsaw, Poland, Nowogrodzka 59 St., 02-006, Warsaw, Poland
Department of Clinical Immunology, Medical University of Warsaw, Poland, Nowogrodzka 59 St., 02-006, Warsaw, Poland
J Pre Clin Clin Res. 2019;13(3):106-109
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Graft-versus-host disease (GVHD) is a serious complication after allogeneic haematopoietic stem cell transplantation (HSCT). Osteopontin (OPN) is a pleiotropic glycoprotein, which plays a significant role in the regulation of biomineralization, wound healing, and cellular immunity. Numerous studies have demonstrated that elevated OPN levels are associated with the pathogenesis of multiple autoimmune and inflammatory conditions. However, its role in GVHD and transplant immunology is poorly understood.
Objective:
The the aim of the study was to investigate the effects of OPN on human peripheral blood mononuclear cells (PBMCs) proliferation in a mixed lymphocyte reaction (MLR).
Material and methods:
PBMCs were isolated from the venous blood of 20 participants. Cell proliferation was examined at the DNA synthesis level by measurements of 3H-thymidine incorporation. Radioactivity was used to calculate the MLR stimulation index (SI). Cell viability was determined using the trypan blue exclusion method.
Results:
OPN enhanced the proliferative response of human alloactivated PBMCs in MLR. Statistically significant results were observed for OPN of 100, 200, 300 and 400 ng/mL (P=0.013, P=0.009; P=0.003 and P < 0.001, respectively). Moreover, OPN increased SI in a dose-dependent way (P=0.011, P=0.007; P=0.002 and P < 0.001, respectively). In addition, this protein did not affect cells viability.
Conclusions:
The results confirm the assumption that OPN may affect the outcome after HSCT; however, future investigation is needed to verify whether it may serve as a valuable predictive and prognostic marker of GVHD.
Graft-versus-host disease (GVHD) is a serious complication after allogeneic haematopoietic stem cell transplantation (HSCT). Osteopontin (OPN) is a pleiotropic glycoprotein, which plays a significant role in the regulation of biomineralization, wound healing, and cellular immunity. Numerous studies have demonstrated that elevated OPN levels are associated with the pathogenesis of multiple autoimmune and inflammatory conditions. However, its role in GVHD and transplant immunology is poorly understood.
Objective:
The the aim of the study was to investigate the effects of OPN on human peripheral blood mononuclear cells (PBMCs) proliferation in a mixed lymphocyte reaction (MLR).
Material and methods:
PBMCs were isolated from the venous blood of 20 participants. Cell proliferation was examined at the DNA synthesis level by measurements of 3H-thymidine incorporation. Radioactivity was used to calculate the MLR stimulation index (SI). Cell viability was determined using the trypan blue exclusion method.
Results:
OPN enhanced the proliferative response of human alloactivated PBMCs in MLR. Statistically significant results were observed for OPN of 100, 200, 300 and 400 ng/mL (P=0.013, P=0.009; P=0.003 and P < 0.001, respectively). Moreover, OPN increased SI in a dose-dependent way (P=0.011, P=0.007; P=0.002 and P < 0.001, respectively). In addition, this protein did not affect cells viability.
Conclusions:
The results confirm the assumption that OPN may affect the outcome after HSCT; however, future investigation is needed to verify whether it may serve as a valuable predictive and prognostic marker of GVHD.
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| eISSN: | 1898-7516 |
| ISSN: | 1898-2395 |
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