RESEARCH PAPER
Metallothionein and manganese concentrations in breast cancer and mastopathic tissues
1
Independent Laboratory for Cell Metabolism, Institute of Rural Health, Lublin, Poland
2
Oncological Centre, Lublin, Poland
3
Department of Bromatology, Medical University, Lublin, Poland
Corresponding author
Bolesław Floriańczyk
Independent Laboratory for Cell Metabolism, Institute of Rural Health, Jaczewskiego 2, 20-950 Lublin, Poland.
Independent Laboratory for Cell Metabolism, Institute of Rural Health, Jaczewskiego 2, 20-950 Lublin, Poland.
J Pre Clin Clin Res. 2011;5(2):63-65
KEYWORDS
ABSTRACT
Metallothionein (MT) takes part in the homeostasis of the metals which are necessary for proper metabolism, and they
also take part in the detoxication of toxic metals from the tissues. Additionally, they protect the tissues from the eff ects
of reactive, oxygen species, radiation, mutagens, and from electrophilic pharmacologic agents used in cancer therapy.
Manganese (Mn) – an essential microelement - seems to be very important during development. Mn takes a part as cofactor
of the enzymes regulating metabolism. This microelement also participates in reduction-oxidation reactions. An example
of such a reaction is the superoxide dismutase (MnSOD) activity, with manganese as the enzyme component. It is thought
that manganese and manganese superoxide dismutase play an important role in the diff erentiating cells. The aim of this
work was to evaluate whether the neoplastic process changes the values of metallothionein and manganese. The examined
material consisted of samples of breast tumour taken during surgery. The level of the metallothionein was determined by the
cadmium- hemoglobin affi nity assay, using the cadmium isotope (109Cd); the concentration of manganese was determined
by atomic absorption spectrometry. It was found that metallothionein and manganese concentration in breast cancer was
higher in comparison with mastopathic lesions of the breast. Neoplastic cells contained more metallothioneins (by 330%)
and more manganese (by 25%). Negative correlations were found between the amount of metallothionein and the level
of manganese in mastopathic lesions (r = -0.57, p < 0.05), and in breast cancer (r = -0.32, p > 0.05). The relatively high level
of metallothionein and manganese in the neoplastic tissues indicates the important role may play in the metabolism of
cancer cells. Negative correlation between the concentration of metallothionein and manganese ions found in mastopathic
tissues and breast cancer indicates that their roles are opposite.
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