RESEARCH PAPER
Glutathione level and glutathione reductase activity in serum of coronary heart disease patients
1
Department of Cardiology with Acute Coronary Syndromes Subunit, Queen Jadwiga Clinical Provincial Hospital No 2 in Rzeszow, Poland
2
Department of Medical Chemistry, Medical University of Lublin, Poland
3
Ist Department of Medical Radiology, Medical University of Lublin, Poland
Corresponding author
Jacek Kurzepa
Department of Medical Chemistry, Department of Medical Chemistry, Medical University of Lublin, Poland, Chodzki 4a, 20-093 Lublin, Poland
Department of Medical Chemistry, Department of Medical Chemistry, Medical University of Lublin, Poland, Chodzki 4a, 20-093 Lublin, Poland
J Pre Clin Clin Res. 2017;11(2):103-105
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Oxidative stress induced by the generation of excess reactive oxygen species is one of the cause of atherosclerosis finally leading to coronary heart disease (CHD). Glutathione reductase (GR), a flavoprotein antioxidant enzyme, regenerates glutathione (GSH) from its oxidized form, which is essential for scavenging of hydrogen peroxide by glutathione peroxidase. The aim of this study was to analyze the activity of GR and GSH level in serum of patients with various stages of CHD.
Material and methods:
Sixty consecutive patients with diagnosis of stable angina (SA, n=20), unstable angina (UA, n=20) and non-fatal myocardial infarction (MI, n=20) together with 20 healthy control subjects were enrolled. Venous blood samples were collected just before the coro-nary catheterization procedure (in study patients). GR activities and GSH concentration was deter-mined using commercially available colorimetric kits.
Results:
The highest GR activity and GSH serum level was noticed in UA patients. However, sta-tistical evaluation showed that only glutathione reductase activity was significantly higher in serum obtained from myocardial infarction patients, compared to stable angina pectoris patients (19.52 ± 13.88 vs 11.63 ± 5.45 nmol/min/ml for MI and SA, respectively; p<0.048). There were no signifi-cant differences in GSH between the CHD patients and controls.
Conclusions:
The elevated activity of glutathione reductase in serum of patients with unstable angi-na pectoris and myocardial infarction suggests the role of antioxidant system acute coronary syn-dromes.
Oxidative stress induced by the generation of excess reactive oxygen species is one of the cause of atherosclerosis finally leading to coronary heart disease (CHD). Glutathione reductase (GR), a flavoprotein antioxidant enzyme, regenerates glutathione (GSH) from its oxidized form, which is essential for scavenging of hydrogen peroxide by glutathione peroxidase. The aim of this study was to analyze the activity of GR and GSH level in serum of patients with various stages of CHD.
Material and methods:
Sixty consecutive patients with diagnosis of stable angina (SA, n=20), unstable angina (UA, n=20) and non-fatal myocardial infarction (MI, n=20) together with 20 healthy control subjects were enrolled. Venous blood samples were collected just before the coro-nary catheterization procedure (in study patients). GR activities and GSH concentration was deter-mined using commercially available colorimetric kits.
Results:
The highest GR activity and GSH serum level was noticed in UA patients. However, sta-tistical evaluation showed that only glutathione reductase activity was significantly higher in serum obtained from myocardial infarction patients, compared to stable angina pectoris patients (19.52 ± 13.88 vs 11.63 ± 5.45 nmol/min/ml for MI and SA, respectively; p<0.048). There were no signifi-cant differences in GSH between the CHD patients and controls.
Conclusions:
The elevated activity of glutathione reductase in serum of patients with unstable angi-na pectoris and myocardial infarction suggests the role of antioxidant system acute coronary syn-dromes.
REFERENCES (18)
1.
Sanchis-Gomar F, Perez-Quilis C, Leischik R, Lucia A. Epidemiology of coronary heart disease and acute coronary syndrome. Ann Transl Med. 2016;4(13):256.
2.
Gresham GA, Howard AN. Atherosclerosis and coronary heart-disease. Lancet. 1960;2(7149):527.
3.
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017;2017:8416763.
4.
Kattoor AJ, Pothineni NVK, Palagiri D, Mehta JL. Oxidative Stress in Atherosclerosis. Curr Atheroscler Rep. 2017;19(11):42.
5.
Griess B, Tom E, Domann F, Teoh-Fitzgerald M. Extracellular superoxide dismutase and its role in cancer. Free Radic Biol Med. 2017;112:464-79.
6.
Wolonciej M, Milewska E, Roszkowska-Jakimiec W. Trace elements as an activator of antioxidant enzymes. Postepy Hig Med Dosw (Online). 2016;70(0):1483-98.
7.
Couto N, Wood J, Barber J. The role of glutathione reductase and related enzymes on cellular redox homoeostasis network. Free Radic Biol Med. 2016;95:27-42.
8.
Forstermann U, Xia N, Li H. Roles of Vascular Oxidative Stress and Nitric Oxide in the Pathogenesis of Atherosclerosis. Circ Res. 2017;120(4):713-35.
9.
Tobin KJ. Stable angina pectoris: what does the current clinical evidence tell us? J Am Osteopath Assoc. 2010;110(7):364-70.
10.
Montalescot G, Sechtem U, Achenbach S, Andreotti F, Arden C, Budaj A, et al. 2013 ESC guidelines on the management of stable coronary artery disease: the Task Force on the management of stable coronary artery disease of the European Society of Cardiology. Eur Heart J. 2013;34(38):2949-3003.
11.
Roffi M, Patrono C, Collet JP, Mueller C, Valgimigli M, Andreotti F, et al. 2015 ESC Guidelines for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation: Task Force for the Management of Acute Coronary Syndromes in Patients Presenting without Persistent ST-Segment Elevation of the European Society of Cardiology (ESC). Eur Heart J. 2016;37(3):267-315.
12.
Thygesen K, Alpert JS, Jaffe AS, Simoons ML, Chaitman BR, White HD, et al. Third universal definition of myocardial infarction. Circulation. 2012;126(16):2020-35.
13.
Deckers JW. Classification of myocardial infarction and unstable angina: a re-assessment. Int J Cardiol. 2013;167(6):2387-90.
14.
Bakan N, Taysi S, Yilmaz O, Bakan E, Kuskay S, Uzun N, et al. Glutathione peroxidase, glutathione reductase, Cu-Zn superoxide dismutase activities, glutathione, nitric oxide, and malondialdehyde concentrations in serum of patients with chronic lymphocytic leukemia. Clin Chim Acta. 2003;338(1-2):143-9.
15.
Patel BP, Raval GN, Rawal RM, Patel JB, Sainger RN, Patel MM, et al. Serum glutathione-S-transferase and glutathione reductase activity in head and neck cancer patients. Neoplasma. 2002;49(4):260-6.
16.
Kaur G, Misra MK, Sanwal GG, Shanker K, Chandra M. Levels of glutathione reductase and glutathione peroxidase of human platelets in unstable angina and myocardial infarction. Boll Chim Farm. 1999;138(8):437-9.
17.
Sapira V, Cojocaru IM, Socoliuc G, Lilios G, Grigorian M, Craiu E, et al. Glutathione reductase levels in patients with unstable angina. Rom J Intern Med. 2011;49(3):197-201.
18.
Uppal N, Uppal V, Uppal P. Progression of Coronary Artery Disease (CAD) from Stable Angina (SA) Towards Myocardial Infarction (MI): Role of Oxidative Stress. J Clin Diagn Res. 2014;8(2):40-3.
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