RESEARCH PAPER
Gel filtration of proteins as a result of heat-treated denaturation of cancer cells homogenate
1
Department of Environmental Hygiene, Institute of Agricultural Medicine, Lublin, Poland
Corresponding author
Bolesław Floriańczyk
Department of Environmental Hygiene, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland.
Department of Environmental Hygiene, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland.
J Pre Clin Clin Res. 2007;1(2):179-181
KEYWORDS
ABSTRACT
The study covered material from patients with brain cancer (astrocytoma G-2). Cancer cells were homogenized, and heat-treated denaturation of cancer cells homogenate was performed. The solution containing heat-stable proteins was applied to a column filled with Sephadex. The results of the study showed that metallothioneins – heat-stable proteins – appear in the eluate in the same fractions as proteins obtained from a rabbit’s liver in the same experimental conditions. The presence of zinc ions was observed in the same fractions as metallothioneins, which indicates that zinc is a stable element in the structure of proteins.
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