RESEARCH PAPER
Effect of N-(p-ethoxycarbonylphenylmethyl) -p-isopropoxyphenylsuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
1
Isobolographic Analysis Laboratory, Institute of Rural Health, Lublin, Poland
2
Isobolographic Analysis Laboratory, Institute of Rural Health, Lublin, Poland; Department of Pathophysiology, Medical University of Lublin, Lublin, Poland
3
Department of Pathophysiology, Medical University of Lublin, Lublin, Poland
4
Mndjoyan’s Institute of Fine Organic Chemistry, National Academy of Sciences, Yerevan, Republic of Armenia
5
Department of Public Health, Institute of Rural Health, Lublin, Poland
Corresponding author
Jarogniew J. Łuszczki
Luszczki, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland Tel.: (+48) 81 718 44 88
Luszczki, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland Tel.: (+48) 81 718 44 88
J Pre Clin Clin Res. 2014;8(1):34-37
KEYWORDS
antiepileptic drugsmaximal electroshock-induced seizuresN-(p-ethoxycarbonyl-phenylmethyl)-p-isopropoxyphenylsuccinimide
ABSTRACT
Introduction and objective:
N-(p-ethoxycarbonylphenylmethyl)-p-isopropoxyphenylsuccinimide (ECPM-IPPS), a new succinimide derivative, on the protective action of four classical antiepileptic drugs (AEDs): carbamazepine (CBZ), phenobarbital (PB), phenytoin (PHT) and valproate (VPA) in the mouse maximal electroshock (MES)-induced tonic seizure model.
Material and Methods:
Tonic hind limb extension (seizure activity) was evoked in adult male albino Swiss mice by a current (sine-wave, 25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via ear-clip electrodes.
Results:
ECPM-IPPS administered (i.p.) at a dose of 150 mg/kg significantly elevated the threshold for electroconvulsions in mice (P<0.05). Lower doses of ECPM-IPPS (50 and 100 mg/kg) had no significant impact on the threshold for electroconvulsions in mice. Moreover, ECPM-IPPS (100 mg/kg) did not significantly affect the anticonvulsant potency of CBZ, PB, PHT and VPA in the MES test in mice.
Conclusions:
ECPM-IPPS elevated the threshold for electroconvulsions in mice in a dose-dependent manner. However, ECPM-IPPS (100 mg/kg) did not affect the anticonvulsant action of various classical AEDs in the mouse MES model, making the combinations of ECPM-IPPS with CBZ, PB, PHT and VPA neutral, from a preclinical point of view.
N-(p-ethoxycarbonylphenylmethyl)-p-isopropoxyphenylsuccinimide (ECPM-IPPS), a new succinimide derivative, on the protective action of four classical antiepileptic drugs (AEDs): carbamazepine (CBZ), phenobarbital (PB), phenytoin (PHT) and valproate (VPA) in the mouse maximal electroshock (MES)-induced tonic seizure model.
Material and Methods:
Tonic hind limb extension (seizure activity) was evoked in adult male albino Swiss mice by a current (sine-wave, 25 mA, 500 V, 50 Hz, 0.2 s stimulus duration) delivered via ear-clip electrodes.
Results:
ECPM-IPPS administered (i.p.) at a dose of 150 mg/kg significantly elevated the threshold for electroconvulsions in mice (P<0.05). Lower doses of ECPM-IPPS (50 and 100 mg/kg) had no significant impact on the threshold for electroconvulsions in mice. Moreover, ECPM-IPPS (100 mg/kg) did not significantly affect the anticonvulsant potency of CBZ, PB, PHT and VPA in the MES test in mice.
Conclusions:
ECPM-IPPS elevated the threshold for electroconvulsions in mice in a dose-dependent manner. However, ECPM-IPPS (100 mg/kg) did not affect the anticonvulsant action of various classical AEDs in the mouse MES model, making the combinations of ECPM-IPPS with CBZ, PB, PHT and VPA neutral, from a preclinical point of view.
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