RESEARCH PAPER
Biochemical bone metabolism markers and morphometric, densitometric and biomechanical properties of femur and tibia in female and gonadectomised male Polish Landrace pigs
1
Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
2
II Department of Radiology, Medical University, Lublin, Poland
3
Department of Conservative Dentistry, Medical University, Lublin, Poland
4
Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
Corresponding author
Marcin R. Tatara
Department of Animal Physiology, University of Life Sciences, Akademicka 12, 20-950 Lublin, Poland.
Department of Animal Physiology, University of Life Sciences, Akademicka 12, 20-950 Lublin, Poland.
J Pre Clin Clin Res. 2012;6(1):14-19
KEYWORDS
ABSTRACT
Estrogens and androgens are critical regulators of bone metabolism and maintain bone mass throughout live in humans
and animals. The aim of the presented study was to compare biochemical bone turnover markers and hormones
influencing bone tissue metabolism and skeletal properties in female and orchidectomised male pigs at slaughter age.
To achieve this aim, femur and tibia from 6-month-old pigs were investigated in terms of morphometric, densitometric
and biomechanical properties. Serum evaluation of osteocalcin (OC), bone-specific alkaline phosphatase (BAP), growth
hormone (GH), insulin-like growth factor-1 (IGF-1) and cotrisol in newborn and 90-day-old pigs was performed. This study
shows a significantly higher growth rate and final body weight gain in orchidectomised males when compared to females
(p<0.05). The obtained results have not shown statistically significant differences in weight, length, volumetric bone mineral
density of the trabecular and cortical bone, vertical and horizontal diameters of the mid-shaft, cross-sectional area, second
moment of inertia, mean relative wall thickness, cortical index, maximum elastic strength and ultimate strength of femur
and tibia in females and orchidectomised males (p>0.05). Bone formation markers such as BAP and OC assessed in serum
were not significantly different between both the investigated groups (p>0.05). Furthermore, serum concentrations of
GH, IGF-1 and cortisol were not gender-differentiated (p>0.05). In conclusion, the differences in body weight gain were
not influenced by gender-differentiated serum concentrations of GH, IGF-1 and cortisol. Lack of significant differences
of morphological, densitometric and biomechanical properties of femur and tibia between the groups was related to
very similar levels of OC, BAP, GH, IGF-1 and cortisol. The data obtained provide novel physiological information on bone
metabolism markers and regulators, as well as properties of femur and tibia in female and gonadectomised male pigs. The
results obtained in this study may therefore be useful in experimental approaches for studying the effects of physiological,
nutritional, pharmacological, toxicological and environmental factors on bone metabolism and skeletal system properties
in pigs.
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