RESEARCH PAPER
Application of high performance thin layer chromatography method for ophthalmological preparations containing anthocyanins fractions
1
Department of Chemistry, Laboratory of Planar Chromatography, Medical Academy, Staszica 6, 20-081, Lublin, Poland
2
II Clinic of Ophthalmology, Medical Academy, Chmielna 3, 20-079, Lublin, Poland
3
Department of Vascular Surgery, Medical Academy, Staszica 3, 20-081, Lublin, Poland
J Pre Clin Clin Res. 2007;1(1):35-38
KEYWORDS
ABSTRACT
The aim of the investigation was to elaborate a fast, easy, and cheap method of analysis of ophthalmological preparations containing anthocyanins fractions. A high performance thin layer chromatography (HPTLC) was applied; the chromatographic conditions were determined experimentally. Eight preparations with extracts of Vaccinium myrtillus L. and Aronia melanocarpa L. as reference material were analyzed. In the optimal chromatographic system, with methyl tert-butyl ether as the modifier of mobile phase, the separation of anthocyanins fractions was very good. The mobile phase enables separation of natural anthocyanins fractions from synthetic pigments (e.g., methylene blue).
REFERENCES (12)
1.
Wiktorowska-Owczarek A, Nowak JZ: Lutein and zeaxanthin – two carotenoids serving as the protection against age-related macular degeneration (AMD). Okulistyka 2004, 4, 48-52.
2.
Cimpoiu C, Hosu A, Hodisan S: Analysis of some steroids by thin-layer chromatography using optimum mobile phases, J Pharm Biomed Anal 2006, 41, 633-637.
3.
Tarnawski M, Depta K, Grejciun D, Szelepin B: HPLC determination of phenolic acids and antioxidant activity in concentrated peat extract – a natural immunomodulator. J Pharm Biomed Anal 2006, 41, 182-188.
4.
Lambri M, Lourdes M, Glories Y, Saucier C: High Performance Thin Layer Chromatography (HPTLC) analysis of red wine pigments. J Planar Chromatogr 2003, 16, 88-94.
5.
da Costa CT, Horton D, Margolis SA: Analysis of anthocyanins in food by liquid chromatography, liquid chromatography-mass spectrometry and capillary electrophoresis. J Chromatogr 2000, A 881, 403-410.
6.
Bridle P, Garcia-Viguera C: Analysis of anthocyanins in strawberries and elderberries. A comparision of capillary zone electrophoresis and HPLC. Food Chem 1997, 59, 299-304.
7.
Bednar P, Tomassi AV, Presutti C, Pavlikova M, Lemar K, Fanami S: Separation of structurally related anthocyanins by MEKC. Chromatographia 2003, 58, 283-291.
8.
Dzido T in Sz. Nyiredy (eds): Planar Chromatography, Springer, Budapest, 2001, pp.386-409.
9.
Matysik G: Modified programmed Multiple Gradient Development (MGD) in the analysis of complex plant extracts. Chromatographia 1996, 43, 39-43.
10.
Matysik G: Thin-Layer Chromatography of anthocyanins with stepwise gradient elution. J Planar Chromatogr 1992, 5, 146-148.
11.
Matysik G, Markowski W, Soczewiński E, Polak B: Computeraided optimalization of stepwise gradient profiles in Thin-Layer Chromatography. Chromatographia 1992, 34, 303-307.
| eISSN: | 1898-7516 |
| ISSN: | 1898-2395 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
