RESEARCH PAPER
Electric charge changes in cross-striated barnacle and rat muscle during contraction
1
The Parker Institute, Frederiksberg Hospital, Copenhagen, Denmark; 2. University Library, Copenhagen, Denmark
2
Department of Ophthalmology, University of Oxford, UK
J Pre Clin Clin Res. 2007;1(2):142-145
KEYWORDS
ABSTRACT
Introduction:
A change in the fixed electrical charge between the rigor and the relaxed condition has been observed previously in the A-band of skeletal muscle. The aim of this study was to observe possible charge changes in the A-band during contraction and relate these to the contractile processes.
Material and Methods:
Donnan potentials were measured in the relaxed state and during Ca-stimulated contractions in skinned rat and barnacle muscle fibres. From the potentials, the fixed electric charges in the A- and the I-bands were calculated
Results:
A rise in negative charge was seen when moving from the relaxed to the contracted state. The A-band charge was more negative during contraction than the charge observed previously in rigor. The I-band charge follows the A-band charge during contraction. Conclusion: The difference in A-band charge in rigor and contracted state may be due to a charge change in both the rod and the head part of the myosin molecule during contraction, but only of the rod part in rigor. This is probably due to the effect of the increasing Pyrophosphate on the I-filament charge, as ATP decreases during contraction.
A change in the fixed electrical charge between the rigor and the relaxed condition has been observed previously in the A-band of skeletal muscle. The aim of this study was to observe possible charge changes in the A-band during contraction and relate these to the contractile processes.
Material and Methods:
Donnan potentials were measured in the relaxed state and during Ca-stimulated contractions in skinned rat and barnacle muscle fibres. From the potentials, the fixed electric charges in the A- and the I-bands were calculated
Results:
A rise in negative charge was seen when moving from the relaxed to the contracted state. The A-band charge was more negative during contraction than the charge observed previously in rigor. The I-band charge follows the A-band charge during contraction. Conclusion: The difference in A-band charge in rigor and contracted state may be due to a charge change in both the rod and the head part of the myosin molecule during contraction, but only of the rod part in rigor. This is probably due to the effect of the increasing Pyrophosphate on the I-filament charge, as ATP decreases during contraction.
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