RESEARCH PAPER
The eff ects of increased inspired oxygen with and without dopamine on lung and diaphragm hydrogen peroxide and apoptosis following hemorrhagic shock
 
More details
Hide details
1
School of Nursing, University of Kansas, Kansas City, USA
 
2
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas, Kansas City, USA
 
 
Corresponding author
Amanda R. Thimmesch   

School of Nursing, University of Kansas, Mail Stop 4043, 3901 Rainbow Blvd., Kansas City, KS 66160.
 
 
J Pre Clin Clin Res. 2010;4(1):5-10
 
KEYWORDS
ABSTRACT
During resuscitation of hemorrhagic shock (HS), clinicians employ high fractions of inspired oxygen (FIO2) to restore maximal oxygen (O2) saturations. Studies indicate that increased FIO2 can be detrimental to cellular function. Our purpose was to determine the FIO2 with and without dopamine (DA) that minimizes hydrogen peroxide (H2O2) production and apoptosis in lung and diaphragm following HS. Sprague-Dawley rats were randomized to FIO2 groups: 0.21, 0.40, 0.60 and 1.0. Controlled HS was elicited by reducing mean arterial pressure to approx. 40 mm Hg. The rats were treated with various FIO2s, with and without DA infusion (10 mcg/kg/min). Hydrogen peroxide was measured using dihydrofl uorescein diacetate. Apoptosis was determined based on nuclear diff erential dye up-take. Compared to 0.21, lung and diaphragm H2O2 and apoptosis were signifi cantly reduced in the 0.40 and 0.60 groups. At an FIO2 of 1.0, H2O2 and apoptosis were greater than at 0.21. With the exception of an FIO2 of 0.40, infusing DA with various FIO2s resulted in H2O2 and apoptosis being signifi cantly decreased. These results indicate that lung and diaphragm H2O2 and apoptosis are aff ected by inspired O2 and DA. Results indicate using an FIO2 of 0.40, with or without DA, is most benefi cial in attenuating tissue damage following HS.
 
REFERENCES (37)
1.
Alam HB, Koustova E, Rhee P: Combat casualty care research: from bench to the battlefi eld. World J Surg 2005, 29(Suppl 1), S7-11.
 
2.
Gutierrez G, Reines HD, Wulf-Gutierrez ME: Clinical review: hemorrhagic shock. Crit Care 2004, 8(5), 373-381.
 
3.
Mauriz JL, Martin Renedo J, Barrio JP, Culebras JM Gonzalez P:Experimental models on hemorragic shock. Nutr Hosp 2007, 22(2),.190-198.
 
4.
Cadenas E: Biochemistry of oxygen toxicity. Annu Rev Biochem 1989, 58, 79-110.
 
5.
Trauma, ACoSCo (2004). Advanced Trauma Life Support for Doctors Student Course Manual Chicago: American College of Surgeons Committee on Trauma.
 
6.
Pietarinen-Runtti P, Raivio KO, Saksela M, Asikainen TM Kinnula VL: Antioxidant enzyme regulation and resistance to oxidants of human bronchial epithelial cells cultured under hyperoxic conditions. Am J Respir Cell Mol Biol 1998, 19(2), 286-292.
 
7.
Klekamp JG, Jarzecka K, Perkett EA: Exposure to hyperoxia decreases the expression of vascular endothelial growth factor and its receptors in adult rat lungs. Am J Pathol 1999, 154(3), 823-831.
 
8.
Khan MM, Ishrat T, Ahmad A, Hoda MN, Khan MB, Khuwaja G, Srivastava P, Raza SS, Islam F, Ahmad S: Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats. Chem Biol Interact 2010, 183(1), 255-263.
 
9.
Miura T, Muraoka S, Ogiso T: Antioxidant activity of adrenergic agents derived from catechol. Biochem Pharmacol 1998, 55(12), 2001-2006.
 
10.
Gassen M, Youdim MB: Free radical scavengers: chemical concepts and clinical relevance. J Neural Transm Suppl 1999, 56,193-210.
 
11.
Goodyear-Bruch C, Simon K, Hall S, Mayo MS, Pierce JD: Comparison of a visual to a computer-assisted technique for detecting apoptosis. Biol Res Nurs 2005, 6(3), 180-186.
 
12.
Atkins JL, Johnson KB, Pearce FJ: Cardiovascular responses to oxygen inhalation after hemorrhage in anesthetized rats: hyperoxic vasoconstriction. Am J Physiol Heart Circ Physiol 2007, 292(2), H776- 785.
 
13.
Souza AL Jr, Poggetti RS, Fontes B, Birolini D: Gut ischemia/reperfusion activates lung macrophages for tumor necrosis factor and hydrogen peroxide production. J Trauma 2000, 49(2), 232-236.
 
14.
Kapoor R, Prasad K: Role of polymorphonuclear leukocytes in cardiovascular depression and cellular injury in hemorrhagic shock and reinfusion. Free Radic Biol Med 1996, 21(5), 609-618.
 
15.
Chen ZH, Saito Y, Yoshida Y, Niki E: Eff ect of oxygen concentration on free radical-induced cytotoxicity. Biosci Biotechnol Biochem 2008, 72(6), 1491-1497.
 
16.
Turrens JF, Freeman BA, Crapo JD: Hyperoxia increases H2O2 release by lung mitochondria and microsomes. Arch Biochem Biophys 1982, 217(2), 411-421.
 
17.
Budzinska K, Ilasz R: Superoxide dismutase mimetic modulates hyperoxic augmentation of the diaphragmatic response to poikilocapnic hypoxia in non-vagotomized rats. J Physiol Pharmacol 2008, 59(Suppl 6), 163-172.
 
18.
Valenca Sdos S, Kloss ML, Bezerra FS, Lanzetti M, Silva FL, Porto LC: [Eff ects of hyperoxia on Wistar rat lungs]. J Bras Pneumol 2007, 33(6), 655-662.
 
19.
Vento M, Moro M, Escrig R, Arruza L, Villar G, Izquierdo I, Roberts LJ 2nd, Arduini A, Escobar JJ, Sastre J, Asensi MA: Preterm resuscitation with low oxygen causes less oxidative stress, infl ammation, and chronic ung disease. Pediatrics 2009, [Epub ahead of print].
 
20.
Lee ES, Smith WE, Quach HT, Jones BD, Santilli SM, Vatassery GT: Moderate hyperoxia (40%) increases antioxidant levels in mouse tissue. J Surg Res 2005, 127(2), 80-84.
 
21.
Gero D, Modis K, Nagy N, Szoleczky P, Toth ZD, Dorman G, Szabo C: Oxidant-induced cardiomyocyte injury: identifi cation of the cytoprotective eff ect of a dopamine 1 receptor agonist using a cellbased high-throughput assay. Int J Mol Med 2007, 20(5), 749-761.
 
22.
Holmes CL, Walley KR: The evaluation and management of shock. Clin Chest Med 2003, 24(4), 775-789.
 
23.
Pierce J, Knight A, Pierce JT, Clancy R, Slusser J: Examination of the diaphragm following the infusion of diff erent fl uid resuscitation therapies after hemorrhagic shock. Am J Crit Care 2009, 18(3).
 
24.
Moran A, Akcan Arikan A, Mastrangelo MA, Wu Y, Yu B, Poli V, Tweardy DJ: Prevention of trauma and hemorrhagic shock-mediated liver apoptosis by activation of stat3alpha. Int J Clin Exp Med 2008, 1(3), 213-247.
 
25.
Shih HC, Wei YH, Lee CH: Diff erential gene expression after hemorrhagic shock in rat lung. J Chin Med Assoc 2005, 68(10), 468- 473.
 
26.
Buccellato LJ, Tso M, Akinci OI, Chandel NS, Budinger GR: Reactive oxygen species are required for hyperoxia-induced Bax activation and cell death in alveolar epithelial cells. J Biol Chem 2004, 279(8), 6753- 6760.
 
27.
Ryter SW, Kim HP, Hoetzel A, Park JW, Nakahira K, Wang X, Choi AM: Mechanisms of cell death in oxidative stress. Antioxid Redox Signal 2007, 9(1), 49-89.
 
28.
Pagano A, Donati Y, Metrailler I, Barazzone Argiroff o C: Mitochondrial cytochrome c release is a key event in hyperoxia-induced lung injury: protection by cyclosporin A. Am J Physiol Lung Cell Mol Physiol 2004, 286(2), L275-283.
 
29.
Childs EW, Udobi KF, Wood JG, Hunter FA, Smalley DM, Cheung LY: In vivo visualization of reactive oxidants and leukocyte-endothelial adherence following hemorrhagic shock. Shock 2002, 18(5), 423- 427.
 
30.
Yamazaki M, Matsuoka T, Yasui K, Komiyama A, Akabane T: Dopamine inhibition of superoxide anion production by polymorphonuclear leukocytes. J Allergy Clin Immunol 1989, 83(5), 967-972.
 
31.
Teramoto S, Tomita T, Matsui H, Ohga E, Matsuse T, Ouchi Y: Hydrogen peroxide-induced apoptosis and necrosis in human lung fi broblasts: protective roles of glutathione. Jpn J Pharmacol 1999, 79(1), 33-40.
 
32.
Supinski GS, Callahan LA: Diaphragmatic free radical generation increases in an animal model of heart failure. J Appl Physiol 2005, 99(3), 1078-1084.
 
33.
Li X, Moody MR, Engel D, Walker S, Clubb FJ Jr, Sivasubramanian N, Mann DL, Reid MB: Cardiac-specifi c overexpression of tumor necrosis factor-alpha causes oxidative stress and contractile dysfunction in mouse diaphragm. Circulation 2000, 102(14), 1690-1696.
 
34.
Zuo L, Clanton TL: Reactive oxygen species formation in the transition to hypoxia in skeletal muscle. Am J Physiol Cell Physiol 2005, 289(1), C207-216.
 
35.
Anzueto A, Brassard JM, Andrade FH, Lawrence RA, et al.: Eff ects of hyperoxia on rat diaphragm function. J Appl Physiol 1994, 77(1), 63- 68.
 
36.
Pierce JD, Clancy RL, Smith-Blair N, Kraft R: Treatment and prevention of diaphragm fatigue using low-dose dopamine. Biol Res Nurs 2002, 3(3), 140-149.
 
37.
Pierce JD, Goodyear-Bruch C, Hall S, Reed GA, et al.: Dopamine alleviation of diaphragm contractile dysfunction and reduction of deoxyribonucleic acid damage in rats. Heart Lung 2008, 37(2), 132-143.
 
eISSN:1898-7516
ISSN:1898-2395
Journals System - logo
Scroll to top